DMEM高糖，細胞培養

DMEM高糖，細胞培養

DMEM是現今細胞培養中比較常見的培養基之一，dmem培養基是建立在MEM培養基的基礎上研究出來的，它與MEM培養基相比較，DMEM培養基中加大了MEM培養基中各種成分的用量，下文主要介紹一下自己配制DMEM的方法。

1 制備新鮮三蒸水或Millipore超純水。

2 稱取所需量的干粉培養基，加入約終體積一半的三蒸水中;若配制一個包裝的培養液，在將整個包裝的干粉倒入三蒸水后，需用水洗包裝袋內面2次，倒入培養液中，以保證所有干粉都溶解成培養液。磁力攪拌或人工攪拌使之*溶解。

3 根據包裝袋上的要求補加所需量的碳酸氫鈉;根據實驗需要，添HEPES(5-20mmol/L)、谷氨酰胺和其他特殊物質。

4 加水定容到終體積。

5 必要時用1 mol/L 鹽酸和1 mol/L 氫氧化鈉調節pH。

6 用無菌0.22um濾膜過濾除菌，分裝于無菌血清瓶中，4℃冰箱保存。

配制好的培養液用前加入100U/mL青霉素和100U/mL鏈霉素，并根據需要加入血清(5%-20%)。 DMEM培養基應用廣泛。基于DMEM培養基中氨基酸、微生物含量豐富，并具有糖酵解中的丙酮酸和微量Fe離子，因此在細胞培養、疫苗生產等方面的應用為常見。

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DMEM高糖，細胞培養