Caspase-9活性檢測(cè)試劑盒

Caspase-9也稱ICE-LAP6或Mch6，可與細(xì)胞*素c和Apaf1形成復(fù)合物被激活，并進(jìn)一步激活細(xì)胞凋亡的最關(guān)鍵酶caspase-3，從而觸發(fā)凋亡級(jí)聯(lián)反應(yīng)。因此，Caspase-9是細(xì)胞凋亡信號(hào)轉(zhuǎn)導(dǎo)過程中重要的上游caspase。
細(xì)胞凋亡屬于程序化細(xì)胞死亡，可見于各種器官和細(xì)胞，在正常發(fā)育、生理、病理過程中對(duì)細(xì)胞和器官的穩(wěn)態(tài)具有十分重要的調(diào)節(jié)作用。Caspase (Cysteine-requiring Aspartate Protease)是參與細(xì)胞凋亡過程的蛋白酶家族，包含10多個(gè)成員。Caspase-9也稱ICE-LAP6或Mch6，可與細(xì)胞*素c和Apaf1形成復(fù)合物被激活，并進(jìn)一步激活細(xì)胞凋亡的最關(guān)鍵酶caspase-3，從而觸發(fā)凋亡級(jí)聯(lián)反應(yīng)。因此，Caspase-9是細(xì)胞凋亡信號(hào)轉(zhuǎn)導(dǎo)過程中重要的上游caspase。其激活可以通過磷酸化進(jìn)行調(diào)控。試劑盒測(cè)定原理基于Caspase-9特異水解其多肽底物Ac-LEHD-pNA (N-acetyl-Leu-Glu-His-Asp-p-nitroanilide)，釋放出游離的硝基ben胺pNA，后者呈黃色在405 nm具有最大吸收峰，用可見光分光光度比色方法測(cè)定。其吸光度值對(duì)應(yīng)于Caspase-9的水解活性。反應(yīng)體系100微升，試劑盒提供的試劑，除標(biāo)準(zhǔn)曲線外可進(jìn)行50或100次樣品測(cè)定。適用：測(cè)定哺乳動(dòng)物組織、細(xì)胞caspase-9活性。

組成與儲(chǔ)存：
1. 裂解緩沖液： 20 ml ，4 oC
2. 反應(yīng)緩沖液： 10 ml ，4 oC
3. 2 mM Ac-LEHD-pNA底物： 0.5 ml，－20 oC避光
4. 10 mM pNA標(biāo)準(zhǔn)品： 0.5 ml，－20 oC避光
以上標(biāo)出的量為100次檢測(cè)，50次檢測(cè)的試劑量減半。試劑常溫運(yùn)輸。到達(dá)后按要求儲(chǔ)存，1年內(nèi)穩(wěn)定。

所需儀器：
酶標(biāo)儀與96孔酶標(biāo)板；或可見光分光光度計(jì)配100μl容積的石英比色杯。
工作波長(zhǎng)：最大吸收波長(zhǎng)405 nm，如不具備也可在400~450 nm范圍內(nèi)測(cè)定。

相關(guān)文獻(xiàn)：
《Overexpression of long noncoding RNA LINC01297 indicates an unfavorable prognosis and promotes tumorigenesis in breast cancer》 作者:Min Jiang,Yu Xiao,Deshui Liu,Na Luo,Qi Gao,Yueyao Guan 期刊:Gene 影響因子:2.638 PMID:29981416
《Long non-coding RNA HOTAIR promotes tumorigenesis and forecasts a poor prognosis in cholangiocarcinoma》 作者:WeiQin,Pengcheng Kang, YiXu, Kaiming Leng, Zhenglong Li, Lining Huang,JianjunGao, YunfuCui & XiangyuZhong 期刊:Scientific Reports 影響因子:4.011 PMID:30111807
《Transient receptor potential ankyrin 1 (trpa1) mediates il-2β-induced apoptosis in rat chondrocytes via calcium overload and mitochondrial dysfunction》 作者:Songjiang Yin, Li Zhang, Liang Ding, Zhengquan Huang, Bo Xu, XiaoChen Li, Peimin Wang & Jun Mao  期刊:Journal of Inflammation 影響因子:2.662 PMID:30564065
《SP1-induced upregulation of lncRNA SPRY4-IT1 exerts oncogenic properties by scaffolding EZH2/LSD1/DNMT1 and sponging miR-101-3p in cholangiocarcinoma》 作者:Yi Xu, Yue Yao, Xingming Jiang, Xiangyu Zhong, Zhidong Wang, Chunlong Li, Pengcheng Kang, Kaiming Leng, Daolin Ji, Zhenglong Li, Lining Huang, Wei Qin & Yunfu Cui  期刊:Journal of Experimental & Clinical Cancer Research 影響因子:5.646 PMID:29642935
《Long non-coding RNA CCAT2 promotes cholangiocarcinoma cells migration and invasion by induction of epithelial-to-mesenchymal transition》 作者:Yi Xu,Yue Yao,Wei Qin,Xiangyu Zhong,Xingming Jiang,Yunfu Cui 期刊:Biomedicine & Pharmacotherapy 影響因子:3.743 PMID:29329034
《Overexpression of hsa_circ_0007534 predicts unfavorable prognosis for osteosarcoma and regulates cell growth and apoptosis by affecting AKT/GSK-4β signaling pathway》 作者:Baoquan Li，Xiaoguang Li 期刊:Biomedicine & Pharmacotherapy 影響因子:3.743 PMID:30142548
《Decreased circular RNA hsa_circ_0001649 predicts unfavorable prognosis in glioma and exerts oncogenic properties in vitro and in vivo》 作者:Yu Wang,Xin Sui,Hong Zhao,Ling Cong,Yu Li,Ting Xin,Mingxing Guo,Wenbo Hao 期刊:Gene 影響因子:2.638 PMID:30016668
《Long non-coding RNA HOTTIP is upregulated in renal cell carcinoma and regulates cell growth and apoptosis by epigenetically silencing of LATS3》 作者:Feifei Peng,Xiaoli Shi,Yin Meng,Bo Dong,Guangchi Xu,Tingting Hou,Yang Shi,Tao Liu 期刊:Biomedicine & Pharmacotherapy 影響因子:3.743 PMID:30021349
《Upregulation of circular RNA hsa_circ_0007534 predicts unfavorable prognosis for NSCLC and exerts oncogenic properties in vitro and in vivo》 作者:Yi Qi,Bingquan Zhang,Junting Wang,Mengna Yao 期刊:Gene 影響因子:2.638 PMID:30017736
《Upregulated long non-coding RNA SPRY4-IT1 predicts dismal prognosis for pancreatic ductal adenocarcinoma and regulates cell proliferation and apoptosis》 作者:Yue Yao,Ping Gao,Lili Chen,Wei Wang,Jinchao Zhang,Qiang Li,Yi,Xu 期刊:Gene 影響因子:2.638 PMID:29551494
《SP1-induced upregulation of lncRNA PANDAR predicts adverse phenotypes in retinoblastoma and regulates cell growth and apoptosis in vitro and in vivo》 作者:Lijie Sheng,Jing Wu,Xuewu Gong,Duo Dong,Xiaoxue Sun 期刊:Gene 影響因子:2.638 PMID:29778422
《Enhanced expression of lncRNA TP73-AS1 predicts unfavorable prognosis for gastric cancer and promotes cell migration and invasion by induction of EMT》 作者:Wei Zhang，Yajuan Zhai，Wenbin Wang，Min Cao，Chunjie Ma 期刊:Gene 影響因子:2.638 PMID:30118890
《A novel prognostic biomarker for pancreatic ductal adenocarcinoma: hsa_circ_0001650》 作者:Yuehong Jiang,Tong Wang,Li Yan,Lijuan Qu 期刊:Gene 影響因子:2.638 PMID:29969694
《Enhanced expression of lncRNA TP73-AS1 predicts adverse phenotypes for cholangiocarcinoma and exerts oncogenic properties in vitro and in vivo》 作者:Yue Yao, Yue Sun,Yuehong Jiang,Lijun Qu,Yi Xu 期刊:Biomedicine & Pharmacotherapy 影響因子:3.743 PMID:29966969
《Upregulated circular RNA circ_0007534 indicates an unfavorable prognosis in pancreatic ductal adenocarcinoma and regulates cell proliferation, apoptosis, and invasion by sponging miR-625 and miR-892b.》 作者:Liguo Hao  Wei Rong  Lianjie Bai  Hongsheng Cui  Shuli Zhang  Yuanchun Li  Datong Chen  Xin Meng 期刊:J. Cell. Biochem. 影響因子:3.448 PMID:30382592
《Upregulated long noncoding RNA LINC01296 indicates a dismal prognosis for pancreatic ductal adenocarcinoma and promotes cell metastatic properties by affecting EMT.》 作者:Qihua Yuan  Yuxia Zhang  Lijun Feng  Yuehong Jiang 期刊:J. Cell. Biochem. 影響因子:3.448 PMID:30203487
 

Caspase-9活性檢測(cè)試劑盒 Caspase-9活性檢測(cè)試劑盒